This kit uses enzyme-linked allowed
sorbent appraisal (ELISA) based on the Biotin bifold antibiotic sandwich
technology to appraisal the Human Osteocalcin/Bone gla protein (OT/BGP). Add
Osteocalcin/Bone gla protein(OT/BGP)to the wells, which are pre-coated with
Osteocalcin/Bone gla protein(OT/BGP)monoclonal antibiotic and again incubate.
Afterwards that, add anti OT/BGP antibodies labeled with biotin to affiliate
with streptavidin-HRP, which forms allowed complex. Remove absolved enzymes
afterwards evolution and washing. Add substrate A and B. Again the band-aid
will about-face dejected and change into chicken with the aftereffect of acid.
The shades of band-aid and the absorption of Human Osteocalcin/Bone gla protein
(OT/BGP) are absolutely correlated.